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Expression levels of the (A) AMPK and (B) SIRT6 genes and their associated proteins were analyzed using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting, and -actin was used as the loading control

Miyazawa T

Although semaglutide is approved for treatment of obesity, tirzepatide and CagriSema are still in clinical trials and not yet approved for obesity

In addition, DMF mediates a time-course-dependent effect to induce DNA hypomethylation on reactive oxygen species (ROS) regulatory genes such as NRF2, CES1-3, GSTA1-5, NOX1-5, NQO1, and TXNRD1 30