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For immunofluorescence, the next day, the secondary antibody and 4,6-diamino-2-phenylpyridine (DAPI) nuclear stain were applied to the paraffin sections, which were then mounted with an anti-quenching solution

Additionally, the study only measured eating behavior during a single laboratory meal at each time point, which may not capture how people eat in their everyday lives, where food choice, timing, and environment play a larger role

If the GLOW three-pathway framework already covers your research question, switching may not be necessary

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