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Information for TB-500 TB-500 is a synthetic peptide derivative of thymosin 4 (T4) whose biological activity remains unconfirmed

For shRNA-mediated COQ2 gene knockdown, oligonucleotides with the following sequences (5-CCATTATAATTCTCAC CTGAA-3

This further results in Ca 2+ inflow and activation of PLA2 (phospholipase A2), which play a key role in the cleavage of secondary fatty and consequently increasing the membrane fluidity, necessary sperm-oocyte fusion [92]

Biotin-MYC was bound to streptavidin-coated 96-well plates (Pierce), and MYC levels were measured by enzyme-linked immunosorbent assay (ELISA) using rabbit anti-MYC antibody and peroxidase-conjugated anti-rabbit antibody followed by a peroxidase colour reaction with TMB ELISA substrate solution (eBioscience)