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The sections were contrast-stained with 2% uranyl acetate followed by lead citrate (Sigma-Aldrich), and imaged in a JEOL 1400 transmission electron microscope (JEOL, Peabody, MA) equipped with a Gatan Orius SC1000 digital CCD camera (Gatan, Pleasanton, CA)

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The LDL receptor family includes the LDL receptor, LDLR-related protein 1 (LRP1), the very-low-density lipoprotein receptor (VLDLR), megalin (LRP2), LRP4, and LRP1b