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Cloudiness, particles, unusual color, or a change in viscosity all indicate degradation

Biol Reprod 109:684692 Sarma UC, Winship AL, Hutt KJ (2020) Comparison of methods for quantifying primordial follicles in the mouse ovary

Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26