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5.10 Primary crypt isolation and intestinal organoid culture Primary intestinal crypts from mouse (ileum, colon) and human (from macroscopically healthy tissue of surgical specimen of the ileum) were isolated by tissue incubation in 2mM EDTA (Fisher) or Gentle Cell Dissociation Reagent (STEMCELL Technologies), respectively, and cultured as described elsewhere (64, 105, 106)

Figure 3)

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However, oral GLP-1s can cause more noticeable digestive side effects, and some research shows that the injectable version may be slightly more effective for weight loss