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Skin preparation and positioning For 4 mm needles (recommended by FIT UK for most adults), a skin pinch is generally not required when injecting at 90 degrees If using longer needles or if you have very little subcutaneous fat, gently pinch a fold of skin between thumb and forefinger This technique helps ensure the needle reaches subcutaneous fat rather than muscle 3

This process usually breaks down beneficial compounds before they reach their cellular destinations

ACKNOWLEDGEMENTS We would like to thank the employees of the Experimental Animal Unit, Faculty of Medicine, Kutahya Health Sciences University for their technical support.

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