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As male mice more robustly develop symptoms of metabolic syndrome than female, only male Ctrl and KO mice were challenged with a 60% high fat diet (D12492, HFD, Research Diets, New Brunswick, USA) for 12 weeks, starting at the age of 5 weeks, before p53 ablation was facilitated by treating the mice with Tamoxifen (100 mg/kg via oral gavage for 5 consecutive days (Molekula group, Munich, Germany) followed by a 1 week wash out period

Bacteriostatic water for injection is used to reconstitute lyophilized (freeze-dried) medications or dilute concentrated injectable drugs according to the drug manufacturer's instructions

The dose is then gradually increased, typically in 2.5 mg increments, based on your goals and tolerance, up to a maximum of 15 mg weekly