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The MassChrom Kit for analysis of amino acids and acylcarnitines from dried blood for newborn screening (57000 F, non-derivatized, Chromsystems Instrument and Chemicals GmbH, Graefelfing, Germany) was used with the following steps: 150 L of a dilution of the Internal Standards (Internal StandardSuccinylacetone:internal Standard, 1:1, v:v) and 75 L of the Extraction BufferSuccinylacetone were added onto the disk

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