For H 2 O 2 treatment, 100 M H 2 O 2 was added to RPMI 1640 growth medium (#1003461059, Sigma Aldrich, United Kingdom), without FBS and phenol red, supplemented with 15 mM HEPES (#1003098482, Sigma Aldrich, USA), 27.9 mM sodium bicarbonate (#101920550, Sigma Aldrich, Japan), 0.2 mM sodium pyruvate (#1002136564, Sigma Aldrich, Japan), 1 U/mL penicillin, and 1 g/mL streptomycin
Nature 628, 424432
form of vitamin E not mentioned in the publication) for over two years significantly delayed functional decline determined by the (in)ability to perform basic activities of daily living and reduced the annual mortality rate in mild and moderate AD patients (111)
Glutathione helps reduce new pigment formation