Among these changes, the KEGG pathway analysis showed that genes involved in the fatty acid beta-oxidation (for example, Acadm , Hadh, and Ehhadh ) and carnitine synthesis (for example, Bbox1 ) were down-regulated in SLC25A45 KO mice relative to control mice, whereas the glycolysis (for example, Hk2 , Gapgh , Pfkfb3 ) and mitochondrial OXPHOS pathways (for example, Ndufa3, Cox7a2, Cpt1a ) were upregulated in SLC25A45 KO mice ( Fig
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Upon review, the VCAM1 assays were considered successful and of good quality, with intermediate-to-strong intensity specific staining of vasculature in both control lung and spleen, with additional VCAM1-specific strong intensity staining of splenic red pulp hematopoietic cells
In addition, some suggested that serum holo-transcobalamin may be a more valid marker of vitamin B-12 status during pregnancy than the markers we used (43)