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HEK293T cells were cultured in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin
Metformin is a medication used to treat type two diabetes and PCOS, and it's the most common medication prescribed to women with PCOS
Astrocytes make up the majority of cells in the human central nervous system (CNS) and are absolutely essential for healthy brain function as they support metabolic, functional, structural, homeostatic, and neuroprotective functions [34,35]
Due to the low number of trials with a low risk of bias and trials that implemented energy restriction alongside their intervention, we did not perform pre-specified sensitivity analyses with trials with a low risk of bias and energy restriction
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