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[DOI] [PMC free article] [PubMed] 41.Cangui-Panchi SP, acato-Toapanta AL, Enrquez-Martnez LJ, Salinas-Delgado GA, Reyes J, Garzon-Chavez D

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The MBP flow-through was then subjected to GST resin, washed with wash buffer containing 2 mM ATP, and bound proteins were eluted with wash buffer containing 50 mM reduced glutathione