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Importantly, heat-induced impairment of spermatogenesis exhibits a progressive worsening with prolonged exposure, underscoring the cumulative threat posed by sustained thermal stress to male reproductive function ( Of the diverse pathological alterations elicited by heat stress, oxidative stress (OS) is widely regarded as a central mechanism responsible for the deterioration of sperm quality ( L-citrulline is a non-essential amino acid synthesized primarily within intestinal enterocytes from glutamine via ornithine transcarbamylase catalysis ( In vivo , L-citrulline is chiefly metabolized through the arginine-nitric oxide (NO) pathway, yielding NO and polyamines as terminal metabolites

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On the contrary, the study of Nazrolu et al

NF-kappaB is a central transcription factor that governs the expression of hundreds of pro-inflammatory genes