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In some embodiments, the loss is caused by a brain injury, e.g

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hCMEC/D3 cultures are permeable to small molecules, such as sucrose or mannitol (25.10 6 cm/s), whereas models based on human pluripotent stem cells are impermeable (0,6.10 6 cm/s) (Eigenmann et al., Junctional Protein, Adhesion Molecules, and Transporters Verifying the expression of relevant cell markers is another key element of model validation