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Aziz Pirani MD and Dr

Floating fraction was collected after 1 h, transferred into non-coated cell-culture flasks and incubated in suspension overnight

Real-time PCR analyses were performed using SYBR Green I PCR kit (Stratagene, La Jolla, CA, USA) via Mx 3000P instrument (Stratagene)

Dynamic transcriptional reprogramming of eosinophils by the skin micromilieu In order to define mechanisms, by which eosinophils exert the observed immunoregulatory effects in chronic cutaneous inflammation, we profiled their transcriptomes at day 14 post-infection using nanowell-based scRNA-seq of viable skin cells and Percoll-enriched blood granulocytes (Fig