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HT1080 cells were first pretreated with these metabolites for 3 h to allow uptake, after thorough washout, ferroptosis was induced by addition of RSL3, a GPX4 inhibitor

As outlined above, studies on non-glutathione disulfide substrates unfortunately regularly lack more detailed kinetic analyses, first, to confirm the efficient turnover of the substrate candidates and, second, to validate the dithiol mechanism