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Most community protocols reconstitute the 80 mg vial with 3.0 mL bacteriostatic water (about 26.7 mg/mL total blend) and use once-daily subcutaneous injections starting at small volumes

The dose is roughly a teaspoon held sublingually for 3060 seconds, then swallowed, taken in the morning on an empty stomach

Methionine Methionine is an essential sulfur-containing amino acid that is critical for protein synthesis, methylation reactions, and the production of sulfur-containing compounds such as cysteine and glutathione

Figure 3 depicts untreated fibroblasts during 24 hours in culture showing that GSH (CMFDA staining-green fluorescence) co-localizes with DNA (Hoechst staining-blue fluorescence)