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siRNA knockdown Macrophages (2.5 10 5 cells/mL) were seeded for 24 h and then were transfected with 10 nM of small interfering RNA (siRNA, GPx1 s804, s805, Thermo Fisher Scientific) or a negative control (Silencer Select siRNA Negative control 4394803, Thermo Fisher Scientific) following the manufacturers instructions

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Oral semaglutide 14 mg was therefore associated with ICERs of GBP 11,006 and GBP 4930 per QALY gained versus empagliflozin 25 mg and sitagliptin 100 mg, respectively, and was considered to be cost effective based on a willingness-to-pay threshold of GBP 20,000 per QALY gained