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Coli containing expressed target fusion protein PBS/EDTA: 1X PBS, 5 mM EDTA, pH 7.4 Lysis buffer: 50 mM Tris, 50 mM NaCl, 5 mM EDTA, 1 g/ml leupeptin, 1 g/ml pepstatin, 0.15 mM PMSF, 1 mM DFP, 1 mM 2-ME, pH 8.0

Second, possible iodine-induced abnormalities should be also considered and further investigated in non-thyroidal tissues, especially those possessing NIS (although not equipped with complete machinery to synthesize thyroid hormones), for example, placenta or breast

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MD simulation supports that the ZIL was concentrated near the surface of cellsZIL molecules, especially their carboxylates, electrostatically interact with the cell membrane, and consequently, ZIL is concentrated ~3 times more densely on the polar surface of the cell membrane than in the bulk state, even at 37 C (Fig