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The samples were incubated with mouse primary monoclonal antibody, anti-Nestin 1:200 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), anti-GAP43 (1:500

After that, the supernatants stored at 80 C and used for antioxidant and biochemical assays

Commonly reported effects include: Nausea or mild stomach upset Diarrhea Fishy or metallic body odor Headaches or dizziness These effects are usually temporary and tend to fade as the body adjusts to supplementation

DWI-R was defined either as an increase in DWI-ASPECTS, a decrease of total DWI signal volume or as partial reversal of the initial DWI lesion irrespective of the final DWI load