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After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample

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A Narrative Review of BPC-157 PubMed 40789979 Emerging Use of BPC-157 in Orthopaedic Sports Medicine PubMed 40756949 Risks of unregulated use of alpha-melanocyte-stimulating hormone analogues PubMed 28266027 Unregulated Peptide Use in the Age of Biohacking: Digital Promotion, Gray-Market Access, and Emerging Public Health Risks PubMed 42437212

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