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6 Integrative roles of CIT, leucine, and exercise: molecular and functional synergy 6.1 Shared and distinct molecular targets (NOmTORAMPK axis) The synergistic interplay of CIT, leucine, and physical exercise constitutes a formidable integrative methodology aimed at reinstating muscle-vascular homeostasis during the aging process by strategically engaging overlapping and complementary molecular pathways

For IF staining, fixed with 4% PFA for 15 min, permeabilized with 0.3% Triton X-100 in PBS for 10 min, and blocked with 5% normal donkey serum for 1 h at room temperature (RT)

BROOD 3.1.4.0: OpenEye Scientific Software, Santa Fe, NM.)

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