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NF-B (p65) DNA-binding activity analysis NF-B (p65) DNA-binding activity in the nuclei of homogenized liver tissues or cultured cells was measured using ELISA kits (Cayman, MI, USA) in accordance with the manufacturers instructions

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Replacement of Q residues with N residues at the polyamination sites had no impact on GSH binding affinity or GST enzymatic activity compared to that of wild-type GST